Bcl-2 / DyLight 350 / 100/D5

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-33315UV (View supplier product page)
Size 0.1 ml
Price $529.00
Antigen Bcl-2
Clone 100/D5
Host Mouse
Isotype IgG1 κ
Conjugate DyLight 350
Target Species Human, Mouse (Negative), Rat (Negative)
Applications WB, FC, ELISA, IHC-Fr, ICC/IF, IHC, IP, IHC-P
Description This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
About Bcl-2 and DyLight 350
Bcl-2 This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
DyLight 350 DyLight™ 350 has an excitation peak of 353 nm and an emission peak of 432 nm. It is spectrally similar to Alexa Fluor™ 350, iFluor® 350, AMCA, and Thioflavin T. DyLight™ 350 is most commonly used in flow cytometery and fluorescence microscopy applications.
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DyLight 350 Excitation and Emission Spectra