CD235a / APC-Vio770 / REA175

Citations: (1)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-120-471 (View supplier product page)
Size 100 tests in 200 µL
Price $365.00
Antigen CD235a
Clone REA175
Host Human
Isotype IgG1
Conjugate APC-Vio770
Target Species Human
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC, Mass Cytometry
Description Clone REA175 recognizes CD235a, commonly known as glycophorin A. CD235a is a single-pass transmembrane glycoprotein and is expressed on mature erythrocytes and erythroid precursor cells. It is one of the major sialoglycoprotein expressed on human red blood cells. | The CD235a (Glycophorin A) antibody has been tested by flow cytometric analysis of human peripheral blood cells according to the protocol. Binding of the antibody to red cells at high and moderate antibody concentration can cause cell agglutination, so it is recommended to titrate the antibody carefully for optimal performance in the assay of interest. | Additional information: Clone REA175 displays negligible binding to Fc receptors. |
About CD235a and APC-Vio770
CD235a Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
APC-Vio770 APC-Vio®770 from Miltenyi is a tandem fluorophore with APC as the donor and has an excitation peak at 652 nm and an emission peak at 775 nm, and is spectrally similar to APC-Cy® 7 (GE Healthcare) APC-H7 (BD Biosciences), APC-eFluor™ 780 (ThermoFisher Scientific), APC-Vio®770 (Miltenyi Biotec), and APC-Fire™ 750 (BioLegend). The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
Citations
PMID 1391951
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APC-Vio770 Excitation and Emission Spectra