CD186 / PE / REA458

Citations: (3)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-123-642 (View supplier product page)
Size 30 tests in 60 µL
Price $115.00
Antigen CD186
Clone REA458
Host Human
Isotype IgG1
Conjugate PE
Target Species Human
Applications FC, Mass Cytometry
Description Clone REA458 recognizes the human CD186 antigen, a multi-pass membrane protein which is also known as C-X-C chemokine receptor type 6 (CXCR6), STRL33, or bonzo. CD186 is expressed on a subset of type 1 polarized peripheral blood CD4+ and CD8+ T cells, γδ T cells, natural killer T cells, natural killer cells, monocytes, and B cells. It is induced in activated peripheral blood lymphocytes. The ligand of CD186 is the chemokine CXCL16. CD186 is used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1. | Additional information: Clone REA458 displays negligible binding to Fc receptors. |
About CD186 and PE
CD186 The protein encoded by this gene is a G protein-coupled receptor with seven transmembrane domains that belongs to the CXC chemokine receptor family. This family also includes CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, and CXCR7. This gene, which maps to the chemokine receptor gene cluster, is expressed in several T lymphocyte subsets and bone marrow stromal cells. The encoded protein and its exclusive ligand, chemokine ligand 16 (CCL16), are part of a signalling pathway that regulates T lymphocyte migration to various peripheral tissues (the liver, spleen red pulp, intestine, lungs, and skin) and promotes cell-cell interaction with dendritic cells and fibroblastic reticular cells. CXCR6/CCL16 also controls the localization of resident memory T lymphocytes to different compartments of the lung and maintains airway resident memory T lymphocytes, which are an important first line of defense against respiratory pathogens. The encoded protein serves as an entry coreceptor used by HIV-1 and SIV to enter target cells, in conjunction with CD4. [provided by RefSeq, Aug 2020]
PE Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Citations
PMID 23596313
PMID 9230441
PMID 20122997
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PE Excitation and Emission Spectra