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CD86 Brilliant Violet 750 (BU63, Mouse) - Reactivity: Human
| Description |
The BU63 monoclonal antibody specifically recognizes CD86 which is also known as B70, B7-2, B7.2, LAB72 or CD28LG2. CD86 is a ~70-80 kDa type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD86 is expressed on monocytes, dendritic cells, Langerhans cells, activated B cells, and memory B cells. Like CD80 (B7-1), CD86 serves as a ligand for CD28 or CD152 (CTLA-4) expressed by T cells and can induce costimulatory or coinhibitory signals, respectively. The BU63 antibody can reportedly block the binding of other human CD86-specific antibodies including the 2331 (FUN-1) (complete block) or IT2.2 (partial block) antibodies. These results suggest that the BU63 antibody may bind to a similar or identical CD86 epitope as the 2331 (FUN-1) antibody but to a different, although spatially-related epitope compared with the IT2.2 antibody. |
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|---|---|---|
| Conjugate | Brilliant Violet 750 | |
| Clone | BU63 | |
| Target Species | Human | |
| Applications | FC | |
| Supplier | BD Biosciences | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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| Isotype |
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Brilliant™ Violet 750 (BV750) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter with a 740 LP dichroic mirror. BV750 has an excitation peak at 405 nm and an emission peak at 750 nm. Due to its unique Stokes shift, no dyes have identical or near identical excitation and emission spectrums. BV750 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV750 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
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