Cytochrome c / Alexa Fluor 405 / 7H8.2C12

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NB100-56503AF405 (View supplier product page)
Size 0.1 ml
Price $549.00
Antigen Cytochrome c
Clone 7H8.2C12
Host Mouse
Isotype IgG2b κ
Conjugate Alexa Fluor 405
Target Species Drosophila, Human, Mouse, Rabbit, Rat
Applications ICC/IF, WB, FC, IHC-P, IHC
Description An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
About Cytochrome c and Alexa Fluor 405
Cytochrome c This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
Alexa Fluor 405 Alexa Fluor™ 405 (AF405, Alexa 405) has an excitation peak at 401 nm and an emission peak at 421 nm and is similar in size, brightness and application to ABflo 405® (ABclonal) and Cascade Blue® (ThermoFisher Scientific). Alexa 405 is commonly used in flow cytometery and fluorescence microscopy applications.
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Alexa Fluor 405 Excitation and Emission Spectra