ErbB2 / Alexa Fluor 350 / ERB2/776

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-48004AF350 (View supplier product page)
Size 0.1 ml
Price $519.00
Antigen ErbB2
Clone ERB2/776
Host Mouse
Isotype IgG1 κ
Conjugate Alexa Fluor 350
Target Species Human
Applications FC, IHC, IHC-P, IF
Description This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
About ErbB2 and Alexa Fluor 350
ErbB2 This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
Alexa Fluor 350 Alexa Fluor™ 350 (AF350, Alexa 350) has an excitation peak at 346 nm and an emission peak at 442 nm, and is spectrally similar in size, brightness and application to AMCA, DyLight™ 350 and CF®350. Alexa 350 is most commonly used in flow cytometery and fluorescence microscopy applications.
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Alexa Fluor 350 Excitation and Emission Spectra