CD45 / DyLight 550 / HI30

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP1-79127R (View supplier product page)
Size 0.1 ml
Price $479.00
Antigen CD45
Clone HI30
Host Mouse
Isotype IgG1 κ
Conjugate DyLight 550
Target Species Human, Primate
Applications WB, FC, IHC, ICC/IF, IHC-P, IHC-Fr, ICFC
Description The mouse monoclonal antibody HI30 recognizes all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. HLDA IV; WS Code N816
About CD45 and DyLight 550
CD45 The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
DyLight 550 DyLight™ 550 has an excitation peak at 562 nm and an emission peak at 576 nm and is spectrally similar to Alexa Fluor™ 546, Alexa Fluor™ 555, Cy3 and TRITC. DyLight™ 550 is most commonly used in flow cytometery and fluorescence microscopy applications.
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DyLight 550 Excitation and Emission Spectra