RB1 / DyLight 550 / 172C1094

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NB100-56598R (View supplier product page)
Size 0.1 ml
Price $509.00
Antigen RB1
Clone 172C1094
Host Mouse
Isotype IgG1
Conjugate DyLight 550
Target Species Human, Mouse
Applications WB, IHC-Fr, IHC-P, IHC
Description The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb. In camptothecin treated HL60, an ~68 kDa band is observed. DRb-p70 exists in vivo as an N-terminally truncated form of Rb (Liu et al.). DRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen. DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. DRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration (Liu et al, 2005).
About RB1 and DyLight 550
RB1 The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
DyLight 550 DyLight™ 550 has an excitation peak at 562 nm and an emission peak at 576 nm and is spectrally similar to Alexa Fluor™ 546, Alexa Fluor™ 555, Cy3 and TRITC. DyLight™ 550 is most commonly used in flow cytometery and fluorescence microscopy applications.
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DyLight 550 Excitation and Emission Spectra