Cytochrome c / DyLight 650 / 7H8.2C12

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NB100-56503C (View supplier product page)
Size 0.1 ml
Price $549.00
Antigen Cytochrome c
Clone 7H8.2C12
Host Mouse
Isotype IgG2b κ
Conjugate DyLight 650
Target Species Drosophila, Human, Mouse, Rabbit, Rat
Applications WB, FC, IHC-P, ICC/IF, IHC
Description An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
About Cytochrome c and DyLight 650
Cytochrome c This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
DyLight 650 DyLight™ 650 is a red-emitting fluorophore that excited by the 640 nm laser and collected using a 670/30 nm bandpass filter. DyLight™ 650 has an excitation peak at 652 nm and an emission peak at 672 nm, and is spectrally similar to Alexa Fluor™ 647 and Cy5. DyLight™ 650 is most commonly used in flow cytometery and fluorescence microscopy applications.
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DyLight 650 Excitation and Emission Spectra