IgM Secondary Antibody / Rhodamine /

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP1-73673 (View supplier product page)
Size 1 mg
Price $289.00
Antigen IgM
Clone
Host Goat
Isotype IgG
Conjugate Rhodamine
Target Species Rat
Applications WB, ICC/IF, FC, FLISA
Description Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
About IgM and Rhodamine
IgM Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
Rhodamine Rhodamine is a generic fluorophore that has an excitation peak at 558 nm and an emission peak at 575 nm. Spectrally, it's similar to Alexa Fluor™ 546.
Images
  IgM Rhodamine Novus Biologicals, a Bio-Techne brand antibody anti target:Rat host:Goat isotype:IgG clone: Western Blot: Goat anti-Rat IgM Secondary Antibody [Rhodamine] [NBP1-73673] - Lane 1: Rat IgG who...

Western Blot: Goat anti-Rat IgM Secondary Antibody [Rhodamine] [NBP1-73673] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
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Rhodamine Excitation and Emission Spectra