CD25 / DyLight 755 / OX-39

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NB100-64761IR (View supplier product page)
Size 0.1 ml
Price $499.00
Antigen CD25
Clone OX-39
Host Mouse
Isotype IgG1
Conjugate DyLight 755
Target Species Rat
Applications FC, ELISA, IHC-Fr, IHC-P, IHC
Description NB100-64761 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.
About CD25 and DyLight 755
CD25 The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020]
DyLight 755 DyLight™ 755 has an excitation peak of 754 nm and an emission peak of 776 nm and is spectrally similar to Alexa Fluor™ 750. DyLight™ 755 is most commonly used in fluorescence micrscopy.
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DyLight 755 Excitation and Emission Spectra