HIF-1 beta / FITC /
HIF-1 beta / FITC /
Product Details
| Supplier | LifeSpan | |
|---|---|---|
| Catalog #: | LS-C694796-100 (View supplier product page) | |
| Size | 100 μl | |
| Price | ||
| Antigen | HIF-1 beta | |
| Clone | ||
| Host | Rabbit | |
| Isotype | IgG | |
| Conjugate | FITC | |
| Target Species | Human, Porcine, Rat | |
| Applications | WB | |
| Description | ARNT / HIF-1-Beta Antibody (aa624-789, FITC) |
About HIF-1 beta and FITC
| HIF-1 beta | This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Citations
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