Carbonic Anhydrase II / FITC /
Carbonic Anhydrase II / FITC /
Product Details
| Supplier | LifeSpan | |
|---|---|---|
| Catalog #: | LS-C691390-200 (View supplier product page) | |
| Size | 200 μl | |
| Price | ||
| Antigen | Carbonic Anhydrase II | |
| Clone | ||
| Host | Rabbit | |
| Isotype | IgG | |
| Conjugate | FITC | |
| Target Species | Human, Monkey, Mouse, Rhesus macaque | |
| Applications | WB | |
| Description | CA2 / Carbonic Anhydrase II Antibody (aa1-260, FITC) |
About Carbonic Anhydrase II and FITC
| Carbonic Anhydrase II | The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Citations
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