Choline Acetyltransferase / FITC /
Choline Acetyltransferase / FITC /
Product Details
| Supplier | LifeSpan | |
|---|---|---|
| Catalog #: | LS-C534954 (View supplier product page) | |
| Size | 100 μl | |
| Price | ||
| Antigen | Choline Acetyltransferase | |
| Clone | ||
| Host | Rabbit | |
| Isotype | IgG | |
| Conjugate | FITC | |
| Target Species | Guinea Pig, Human, Porcine, Rat | |
| Applications | ELISA, IHC | |
| Description | CHAT Antibody (FITC) |
About Choline Acetyltransferase and FITC
| Choline Acetyltransferase | This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010] | |
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| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Citations
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