IgM Secondary Antibody / Rhodamine /

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP1-73059 (View supplier product page)
Size 1 mg
Price $209.00
Antigen IgM
Clone
Host Goat
Isotype IgG
Conjugate Rhodamine
Target Species Human
Applications ICC/IF, FC, FLISA
Description Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against other human heavy or light chain proteins.
About IgM and Rhodamine
IgM Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
Rhodamine Rhodamine is a generic fluorophore that has an excitation peak at 558 nm and an emission peak at 575 nm. Spectrally, it's similar to Alexa Fluor™ 546.
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Rhodamine Excitation and Emission Spectra