IgG Fc Fragment Secondary Antibody / Rhodamine /

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP1-73329 (View supplier product page)
Size 2 mg
Price $259.00
Antigen IgG Fc Fragment
Clone
Host Goat
Isotype IgG
Conjugate Rhodamine
Target Species Rabbit
Applications WB, ICC/IF, FC, FLISA
Description This antibody was pre-adsorbed against Human Serum Proteins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab) or Human Serum Proteins.
Images
  IgG Fc Fragment Rhodamine Novus Biologicals, a Bio-Techne brand antibody anti target:Rabbit host:Goat isotype:IgG clone: Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [Rhodamine] (Pre-absorbed) [NBP1-73329] ...

Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [Rhodamine] (Pre-absorbed) [NBP1-73329] - Goat anti-Rabbit IgG Fc Secondary Antibody [Rhodamine] [NBP1-73329] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
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Rhodamine Excitation and Emission Spectra