FluoroFinder

	IL-17F	The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]
	Alexa Fluor 647	Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.

		PdBU/ionomycin-stimulated (5 hours) Th17-polarized CD4⁺ T cells from C57BL/6 mouse lymph nodes surface stained with CD4 (GK1.5) PE, then intracellularly stained with 8F5.1A9 Alexa Fluor® 647
		PdBU/ionomycin-stimulated (5 hours) Th17-polarized CD4⁺ T cells from C57BL/6 mouse lymph nodes intracellularly stained with IL-17A (TC11-18H10.1) PE and 8F5.1A9 Alexa Fluor® 647