IL-4 / Brilliant Violet 421 / 11B11
IL-4 / Brilliant Violet 421 / 11B11
Citations:
(14)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 504119 (View supplier product page) | |
| Size | 125UL | |
| Price | $193.00 | |
| Antigen | IL-4 | |
| Clone | 11B11 | |
| Host | Rat | |
| Isotype | IgG1 κ | |
| Conjugate | Brilliant Violet 421 | |
| Target Species | Mouse | |
| Applications | ICS | |
| Description | Brilliant Violet 421 anti-mouse IL-4 |
About IL-4 and Brilliant Violet 421
| IL-4 | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020] | |
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| Brilliant Violet 421 | Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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PMA+ionomycin-stimulated (6 hours, in presence of brefeldin A) Th2-polarized C57BL/6 T cells were surface stained with CD4 APC and then intracellularly stained with IL-4 ( clone 11B11) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom). |
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Citations
| PMID 17911622 | ||
|---|---|---|
| PMID 18941115 | ||
| PMID 20512127 | ||
| Additional Sources |
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Reviews & Ratings
Brilliant Violet 421 Excitation and Emission Spectra
$193.00
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