H2AFY2 / FITC /
H2AFY2 / FITC /
Product Details
| Supplier | LifeSpan | |
|---|---|---|
| Catalog #: | LS-C463242 (View supplier product page) | |
| Size | 100 μl | |
| Price | ||
| Antigen | H2AFY2 | |
| Clone | ||
| Host | Rabbit | |
| Isotype | IgG | |
| Conjugate | FITC | |
| Target Species | Bat, Bovine, Canine, Chimpanzee, Gibbon, Guinea Pig, Human, Monkey, Mouse, Porcine, Rabbit, Rat | |
| Applications | WB | |
| Description | H2AFY2 Antibody (aa143-192, FITC) |
About H2AFY2 and FITC
| H2AFY2 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and may participate in stable X chromosome inactivation. [provided by RefSeq, Oct 2015] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Citations
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