CD8a / FITC / SK1

Citations: (66)
Product Details
Supplier BioLegend
Catalog #: 344704 (View supplier product page)
Size 100T
Price $242.00
Antigen CD8a
Clone SK1
Host Mouse
Isotype IgG1 κ
Conjugate FITC
Target Species Cynomolgus macaque, Human, Rhesus macaque
Applications FC
Description FITC anti-human CD8
About CD8a and FITC
CD8a The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Images
  CD8a FITC BioLegend antibody anti target:Cynomolgus macaque, Human, Rhesus macaque host:Mouse isotype:IgG1 κ clone:SK1

Human peripheral blood lymphocytes stained with SK1 FITC
Citations
PMID 21402742
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FITC Excitation and Emission Spectra