CD40 / Alexa Fluor 647 / 5C3

Citations: (5)
Product Details
Supplier BioLegend
Catalog #: 334312 (View supplier product page)
Size 100T
Price $265.00
Antigen CD40
Clone 5C3
Host Mouse
Isotype IgG1 κ
Conjugate Alexa Fluor 647
Target Species Cynomolgus macaque, Human, Rhesus macaque
Applications FC
Description Alexa Fluor 647 anti-human CD40
About CD40 and Alexa Fluor 647
CD40 This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
Alexa Fluor 647 Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
Images
  CD40 Alexa Fluor 647 BioLegend antibody anti target:Cynomolgus macaque, Human, Rhesus macaque host:Mouse isotype:IgG1 κ clone:5C3

Human peripheral blood lymphocytes stained with 5C3 Alexa Fluor® 647
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Alexa Fluor 647 Excitation and Emission Spectra