PD-1 / FITC / EH12.2H7
PD-1 / FITC / EH12.2H7
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 329903 (View supplier product page) | |
| Size | 25T | |
| Price | $106.00 | |
| Antigen | PD-1 | |
| Clone | EH12.2H7 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | FITC | |
| Target Species | Human | |
| Applications | FC | |
| Description | FITC anti-human CD279 (PD-1) |
About PD-1 and FITC
| PD-1 | Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Images
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PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) FITC (filled histogram) or mouse IgG1, κ FITC (open histogram). |
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Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) FITC and CD3 (clone UCHT1) PerCP/Cy5.5. |
Citations
| PMID 18829748 | ||
|---|---|---|
| PMID 18981087 | ||
| PMID 19553327 | ||
| PMID 19889785 | ||
| PMID 20100932 | ||
| PMID 21398606 | ||
| PMID 21562156 | ||
| Additional Sources |
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Reviews & Ratings
FITC Excitation and Emission Spectra
$106.00
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