AKT1S1 (pT246) / FITC /
AKT1S1 (pT246) / FITC /
Product Details
| Supplier | LifeSpan | |
|---|---|---|
| Catalog #: | LS-C450114 (View supplier product page) | |
| Size | 100 μl | |
| Price | ||
| Antigen | AKT1S1 (pT246) | |
| Clone | ||
| Host | Rabbit | |
| Isotype | IgG | |
| Conjugate | FITC | |
| Target Species | Bat, Bovine, Chimpanzee, Equine, Gibbon, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rat | |
| Applications | WB | |
| Description | AKT1S1 / PRAS40 Antibody (aa202-251, FITC) |
About AKT1S1 (pT246) and FITC
| AKT1S1 (pT246) | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Citations
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