CD107a / FITC / H4A3

Citations: (27)
Ratings:
View
Product Details
Supplier BioLegend
Catalog #: 328605 (View supplier product page)
Size 25T
Price $98.00
Antigen CD107a
Clone H4A3
Host Mouse
Isotype IgG1 κ
Conjugate FITC
Target Species Human
Applications FC
Description FITC anti-human CD107a (LAMP-1)
About CD107a and FITC
CD107a The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Images
  CD107a FITC BioLegend antibody anti target:Human host:Mouse isotype:IgG1 κ clone:H4A3

Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) FITC (red histogram) or mouse IgG1, κ FITC (green histogram).

Citations
FITC Excitation and Emission Spectra