FluoroFinder

	CD14	The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2020]
	Alexa Fluor 647	Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.

		Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA) and then stained with 5 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (red) and 5 µg/ml CD3 (clone OKT3) Brilliant Violet 421™ (blue) for 30 minutes at room temperature. The image was captured by 40X objective.
		Human peripheral blood monocytes stained with HCD14 alexa Fluor® 647