FluoroFinder

	Supplier	BioLegend
	Catalog #:	324412 (View supplier product page)
	Size	100T
	Price	$293.00
	Antigen	ErbB2
	Clone	24D2
	Host	Mouse
	Isotype	IgG1 κ
	Conjugate	Alexa Fluor 647
	Target Species	Human
	Applications	FC, ICC
	Description	Alexa Fluor 647 anti-human CD340 (erbB2/HER-2)

	ErbB2	This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
	Alexa Fluor 647	Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.

		BT474 breast cancer cell line was stained with 2 µg/mL anti-human CD340/Her-2 Alexa Fluor® 647 and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute
		Human breast cancer cell line (MCF-7) stained with 24D2 Alexa Fluor® 647