CD40L / Brilliant Violet 605 / 24-31

Citations: (3)
Product Details
Supplier BioLegend
Catalog #: 310825 (View supplier product page)
Size 25T
Price $248.00
Antigen CD40L
Clone 24-31
Host Mouse
Isotype IgG1 κ
Conjugate Brilliant Violet 605
Target Species Human
Applications FC
Description Brilliant Violet 605 anti-human CD154
About CD40L and Brilliant Violet 605
CD40L The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Brilliant Violet 605 Brilliant™ Violet 605 (BV605) is an orange/red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 610/20 bandpass filter. BV605 has an excitation peak at 405 nm and an and emission peak at 603 nm. BV605 is most commonly used in flow cytometry and is spectrally similar to SuperBright 600 (Thermo Fisher), SuperNova v605 (Beckman Coulter) and StarBright Violet 610 (Bio-Rad). BV605 is considered to be relatively bright and should be used on antigens of moderate to low abudance. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Images
  CD40L Brilliant Violet 605 BioLegend antibody anti target:Human host:Mouse isotype:IgG1 κ clone:24-31

6-hour PMA+ionomycin-stimulated human peripheral blood lymphocytes were stained with CD154 (clone 24-31) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).

Citations
PMID 17626840
PMID 21242519
Additional
Sources
Reviews & Ratings
Brilliant Violet 605 Excitation and Emission Spectra