CD80 / Brilliant Violet 605 / 2D10

Citations: (12)
Product Details
Supplier BioLegend
Catalog #: 305225 (View supplier product page)
Size 25T
Price $248.00
Antigen CD80
Clone 2D10
Host Mouse
Isotype IgG1 κ
Conjugate Brilliant Violet 605
Target Species Human
Applications FC
Description Brilliant Violet 605 anti-human CD80
About CD80 and Brilliant Violet 605
CD80 The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
Brilliant Violet 605 Brilliant™ Violet 605 (BV605) is an orange/red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 610/20 bandpass filter. BV605 has an excitation peak at 405 nm and an and emission peak at 603 nm. BV605 is most commonly used in flow cytometry and is spectrally similar to SuperBright 600 (Thermo Fisher), SuperNova v605 (Beckman Coulter) and StarBright Violet 610 (Bio-Rad). BV605 is considered to be relatively bright and should be used on antigens of moderate to low abudance. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Images
  CD80 Brilliant Violet 605 BioLegend antibody anti target:Human host:Mouse isotype:IgG1 κ clone:2D10

Human B-cell Burkitt's lymphoma cell line Raji was stained with CD80 (clone 2D10) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).

Citations
PMID 18316378
PMID 22312112
Additional
Sources
Reviews & Ratings
Brilliant Violet 605 Excitation and Emission Spectra