CD62L / FITC / DREG-56

Citations: (16)
Product Details
Supplier BioLegend
Catalog #: 304804 (View supplier product page)
Size 100T
Price $191.00
Antigen CD62L
Clone DREG-56
Host Mouse
Isotype IgG1 κ
Conjugate FITC
Target Species Human
Applications FC
Description FITC anti-human CD62L
About CD62L and FITC
CD62L This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Images
  CD62L FITC BioLegend antibody anti target:Human host:Mouse isotype:IgG1 κ clone:DREG-56

Human peripheral blood lymphocytes stained with DREG-56 FITC
Citations
PMID 18490743
PMID 18941247
PMID 19849846
PMID 20512127
PMID 21976773
PMID 23948971
PMID 23999103
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FITC Excitation and Emission Spectra