CD20 / Brilliant Violet 421 / 2H7
CD20 / Brilliant Violet 421 / 2H7
Citations:
(21)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 302330 (View supplier product page) | |
| Size | 100T | |
| Price | $381.00 | |
| Antigen | CD20 | |
| Clone | 2H7 | |
| Host | Mouse | |
| Isotype | IgG2b κ | |
| Conjugate | Brilliant Violet 421 | |
| Target Species | Cynomolgus macaque, Human, Rhesus macaque | |
| Applications | IHC-Fr, FC | |
| Description | Brilliant Violet 421 anti-human CD20 |
About CD20 and Brilliant Violet 421
| CD20 | This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008] | |
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| Brilliant Violet 421 | Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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Human peripheral blood lymphocytes were stained with CD19 PE and CD20 (clone 2H7) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom). |
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Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-human CD20 (clone 2H7) Brilliant Violet 421™ (blue) and 10 µg/ml of anti-human CD3 (clone UCHT1) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective. |
Citations
| PMID 24958280 | ||
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| Additional Sources |
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Reviews & Ratings
Brilliant Violet 421 Excitation and Emission Spectra
$381.00
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