CD19 / Alexa Fluor 488 / HIB19
CD19 / Alexa Fluor 488 / HIB19
Citations:
(19)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 302219 (View supplier product page) | |
| Size | 100T | |
| Price | $268.00 | |
| Antigen | CD19 | |
| Clone | HIB19 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Alexa Fluor 488 | |
| Target Species | Human | |
| Applications | FC, ICC | |
| Description | Alexa Fluor 488 anti-human CD19 |
About CD19 and Alexa Fluor 488
| CD19 | This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020] | |
|---|---|---|
| Alexa Fluor 488 | Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. Alexa 488 and is similar in size, brightness and application to DyLight™ 488, iFluor® 488 and CF®488A. |
Images
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Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (yellow), 10 µg/ml CD19 (clone HIB19) Alexa Fluor® 488 (green), and 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 594 (red) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective. |
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Human peripheral blood lymphocytes stained with HIB19 Alexa Fluor® 488 |
Citations
| PMID 19155503 | ||
|---|---|---|
| PMID 20015889 | ||
| PMID 20410262 | ||
| Additional Sources |
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Reviews & Ratings
Alexa Fluor 488 Excitation and Emission Spectra
$268.00
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