Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD3 (clone UCHT1) Brilliant Violet 421™ (blue) and 5 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.

Human frozen tonsil tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 5 µg/mL of Brilliant Violet 421™ anti-human CD3 antibody (blue) and Alexa Fluor® 647 anti-human CD19 antibody (red) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.