PD-1 / Brilliant Violet 421 / 29F.1A12
PD-1 / Brilliant Violet 421 / 29F.1A12
Citations:
(26)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 135217 (View supplier product page) | |
| Size | 125UL | |
| Price | $204.00 | |
| Antigen | PD-1 | |
| Clone | 29F.1A12 | |
| Host | Rat | |
| Isotype | IgG2a κ | |
| Conjugate | Brilliant Violet 421 | |
| Target Species | Mouse | |
| Applications | FC, Spatial Biology | |
| Description | Brilliant Violet 421 anti-mouse CD279 (PD-1) |
About PD-1 and Brilliant Violet 421
| PD-1 | Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020] | |
|---|---|---|
| Brilliant Violet 421 | Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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Con-A stimulated C57BL/6 splenocytes (3 days) were stained with CD3 APC and CD279 (clone 29F.1A12) Brilliant Violet 421™ (top), or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom). |
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Citations
| PMID 20453843 | ||
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Reviews & Ratings
Brilliant Violet 421 Excitation and Emission Spectra
$204.00
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