CD40 / PE-Cy7 / 3/23

Citations: (20)
Product Details
Supplier BioLegend
Catalog #: 124622 (View supplier product page)
Size 100 μg
Price $347.00
Antigen CD40
Clone 3/23
Host Rat
Isotype IgG2a κ
Conjugate PE-Cy7
Target Species Mouse
Applications FC
Description PE/Cyanine7 anti-mouse CD40
About CD40 and PE-Cy7
CD40 This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
PE-Cy7 PE-Cyanine®7 (PE-Cy7, RPE-Cy7) is a far red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy7. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy7, which emits light that can be captured with a 780/60 nm bandpass filter. PE-CY7 has an excitation peak at 565 nm and an emission peak at 778 nm, and is a suitable alternative to PE-Vio®770 (Miltenyi) and cFluor BYG781 (Cytek).
Images
  CD40 PE-Cy7 BioLegend antibody anti target:Mouse host:Rat isotype:IgG2a κ clone:3/23

BALB/c splenocytes stained with CD40 (clone 3/23) PE/Cy7 (filled histogram), or rat IgG2a, κ PE/Cy7 isotype control (open histogram).
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PE-Cy7 Excitation and Emission Spectra