CD122 / FITC / TM-beta1

Citations: (4)
Product Details
Supplier BioLegend
Catalog #: 123208 (View supplier product page)
Size 500 μg
Price $297.00
Antigen CD122
Clone TM-beta1
Host Rat
Isotype IgG2b κ
Conjugate FITC
Target Species Mouse
Applications FC
Description FITC anti-mouse CD122 (IL-2Rbeta)
About CD122 and FITC
CD122 The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. The use of alternative promoters results in multiple transcript variants encoding the same protein. The protein is primarily expressed in the hematopoietic system. The use by some variants of an alternate promoter in an upstream long terminal repeat (LTR) results in placenta-specific expression. [provided by RefSeq, Sep 2016]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Images
  CD122 FITC BioLegend antibody anti target:Mouse host:Rat isotype:IgG2b κ clone:TM-beta1

C57BL/6 mouse splenocytes stained with TM-β1 FITC
Citations
Additional
Sources
Reviews & Ratings
Submit a Review

FITC Excitation and Emission Spectra