F4/80 / Brilliant Violet 421 / BM8
F4/80 / Brilliant Violet 421 / BM8
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 123131 (View supplier product page) | |
| Size | 125UL | |
| Price | $204.00 | |
| Antigen | F4/80 | |
| Clone | BM8 | |
| Host | Rat | |
| Isotype | IgG2a κ | |
| Conjugate | Brilliant Violet 421 | |
| Target Species | Mouse | |
| Applications | FC, IHC-Fr, Spatial Biology | |
| Description | Brilliant Violet 421 anti-mouse F4/80 |
About F4/80 and Brilliant Violet 421
| F4/80 | This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] | |
|---|---|---|
| Brilliant Violet 421 | Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with F4/80 (clone BM8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). |
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C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 2 µg/ml of anti-mouse F4/80 (clone BM8) Brilliant Violet 421™ (Blue) and anti-mouse CD8a (clone 53-6.7) Alexa Fluor® 647 (red) overnight at 4°C. The image was captured with a 10X objective. |
Citations
| PMID 18372338 | ||
|---|---|---|
| PMID 19509298 | ||
| PMID 23554311 | ||
| PMID 24657529 | ||
| PMID 24711449 | ||
| PMID 25589074 | ||
| Additional Sources |
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Reviews & Ratings
Brilliant Violet 421 Excitation and Emission Spectra
$204.00
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