IgM / DyLight 550 /
IgM / DyLight 550 /
Product Details
| Supplier | LifeSpan | |
|---|---|---|
| Catalog #: | LS-C355811 (View supplier product page) | |
| Size | 500 μg | |
| Price | ||
| Antigen | IgM | |
| Clone | ||
| Host | Goat | |
| Isotype | IgG | |
| Conjugate | DyLight 550 | |
| Target Species | Human | |
| Applications | FC, ICC, IF, IHC, IP, WB | |
| Description | Goat Anti-Human IgM Secondary Antibody (DY550) |
About IgM and DyLight 550
| IgM | Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010] | |
|---|---|---|
| DyLight 550 | DyLight™ 550 has an excitation peak at 562 nm and an emission peak at 576 nm and is spectrally similar to Alexa Fluor™ 546, Alexa Fluor™ 555, Cy3 and TRITC. DyLight™ 550 is most commonly used in flow cytometery and fluorescence microscopy applications. |
Citations
| Additional Sources |
|
|---|
