FluoroFinder

	Supplier	BioLegend
	Catalog #:	104725 (View supplier product page)
	Size	125UL
	Price	$204.00
	Antigen	CD80
	Clone	16-10A1
	Host	Armenian Hamster
	Isotype	IgG
	Conjugate	Brilliant Violet 421
	Target Species	Mouse
	Applications	FC, IHC-Fr, IHC-Fr
	Description	Brilliant Violet 421 anti-mouse CD80

	CD80	The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
	Brilliant Violet 421	Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad.

		LPS-stimulated (day 3) C57BL/6 mouse splenocytes stained with CD80 (clone 16-10A1) Brilliant Violet 421™ (filled histogram) or Armenian hamster IgG Brilliant Violet 421™ isotype control (open histogram).
		C57BL/6 mouse frozen thymus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD80 (clone 16-10A1) Brilliant VioletÂ® 421 (purple), 10 µg/ml of CD90.2 (clone 30-H12) Alexa Fluor® 647 (red) and 10 µg/ml of CD326 (clone G8.8) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured by 10X objective.