CD69 / Brilliant Violet 421 / H1.2F3

Citations: (26)
Product Details
Supplier BioLegend
Catalog #: 104527 (View supplier product page)
Size 125UL
Price $193.00
Antigen CD69
Clone H1.2F3
Host Armenian Hamster
Isotype IgG
Conjugate Brilliant Violet 421
Target Species Mouse
Applications FC
Description Brilliant Violet 421 anti-mouse CD69
About CD69 and Brilliant Violet 421
CD69 This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]
Brilliant Violet 421 Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad.
Images
  CD69 Brilliant Violet 421 BioLegend antibody anti target:Mouse host:Armenian Hamster isotype:IgG clone:H1.2F3

PMA+ionomycin-stimulated (6 hours) C57BL/6 mouse splenocytes were stained with CD69 (clone H1.2F3) Brilliant Violet 421™ (filled histogram) or Armenian hamster IgG Brilliant Violet 421™ isotype control (open histogram).

Citations
Reviews & Ratings
Brilliant Violet 421 Excitation and Emission Spectra