CD28 / PerCP-Cy5.5 / 37.51

Citations: (4)
Product Details
Supplier BioLegend
Catalog #: 102113 (View supplier product page)
Size 25 μg
Price $118.00
Antigen CD28
Clone 37.51
Host Syrian Hamster
Isotype IgG
Conjugate PerCP-Cy5.5
Target Species Mouse
Applications FC
Description PerCP/Cyanine5.5 anti-mouse CD28
About CD28 and PerCP-Cy5.5
CD28 The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
PerCP-Cy5.5 PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.
Images
  CD28 PerCP-Cy5.5 BioLegend antibody anti target:Mouse host:Syrian Hamster isotype:IgG clone:37.51

C57BL/6 mouse splenocytes were stained with CD3 APC and CD28 (clone 37.51) PerCP/Cyanine5.5 (left) or Syrian Hamster IgG PerCP/Cyanine5.5 isotype control (right).

Citations
Reviews & Ratings
PerCP-Cy5.5 Excitation and Emission Spectra