C57BL/6 mouse splenocytes were stained with CD19 FITC and CD3ε (clone 145-2C11) Brilliant Violet 421™ (above) or Armenian hamster IgG Brilliant Violet 421™ isotype control (below).

C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD3 (clone 145-2C11) Brilliant Violet 421™ (blue) and 5 µg/ml CD19 (clone 6D5) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.