IgG Secondary Antibody / DyLight 594 / Poly4055

Citations: (6)
Product Details
Supplier BioLegend
Catalog #: 405504 (View supplier product page)
Size 100 μg
Price $106.00
Antigen IgG
Clone Poly4055
Host Goat
Isotype IgG
Conjugate DyLight 594
Target Species Armenian Hamster
Applications ICC
Description DyLight 594 Goat anti-hamster (Armenian) IgG
About IgG and DyLight 594
IgG This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
DyLight 594 DyLight™ 594 has an excitation peak at 593 nm and an emission peak at 618 nm and is spectrally similar to Alexa Fluor™ 594 and Texas Red. DyLight™ 594 is most commonly used in flow cytometery and fluorescence microscopy applications.
Images
  IgG DyLight 594 BioLegend antibody anti target:Armenian Hamster host:Goat isotype:IgG clone:Poly4055

Mouse splenocytes were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 10 µg/mL anti-mouse CD3ε Antibody (145-2C11) Antibody and followed by DyLight™ 594 goat anti-hamster IgG (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

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DyLight 594 Excitation and Emission Spectra