Helios Monoclonal / PE-eFluor 610 / 22F6

Citations: (6)
Product Details
Supplier Thermo Fisher Scientific
Catalog #: 61-9883-42 (View supplier product page)
Size 100 Tests
Price $499.00
Antigen Helios
Clone 22F6
Host Armenian Hamster
Isotype IgG
Conjugate PE-eFluor 610
Target Species Canine, Human, Mouse
Applications FC
Description HELIOS Monoclonal Antibody (22F6), PE-eFluor 610, eBioscience
About Helios and PE-eFluor 610
Helios This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
PE-eFluor 610 PE-eFluor™ 610 (PE-eF610, RPE-eF610) from Thermo Fisher Scientific is an orange-emitting tandem fluorophore that combines pycoerythrin (PE) and eFluor™ 610. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, eF610, which emitts light that can be captured with a 610/20 nm bandpass filter. PE-eF610 has an excitation peak at 565 nm and an emission peak at 607 nm, and is suitable alternative to PE-Texas Red®. PE-eF610 is mainly used for flow cytometry. This tandem is often a great option because of PE is excitable by multiple lasers due to its broad excitation spectrum and the eFluor™ 610 acceptor molecule emits brightly in the Orange/Red spectrum. Many cytometers are commonly set up with the 610/20 bandpass filter that can detect this fluorophore's emission. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
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PE-eFluor 610 Excitation and Emission Spectra