CD28 Monoclonal / FITC / CD28.2

Citations: (12)
Product Details
Supplier Thermo Fisher Scientific
Catalog #: 11-0289-42 (View supplier product page)
Size 100 Tests
Price $209.00
Antigen CD28
Clone CD28.2
Host Mouse
Isotype IgG1 κ
Conjugate FITC
Target Species Human
Applications FC
Description CD28 Monoclonal Antibody (CD28.2), FITC, eBioscience
About CD28 and FITC
CD28 The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra