IgM / APC / PJ2-22H3

Product Details
Supplier Miltenyi Biotec
Catalog #: 130-122-959 (View supplier product page)
Size 30 tests in 60 µL
Price $145.00
Antigen IgM
Clone PJ2-22H3
Host Mouse
Isotype IgG1
Conjugate APC
Target Species Human
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC, Mass Cytometry
Description IgM is the first immunoglobulin produced by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre–B cells. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation. It reacts with the soluble as well as membrane-bound form of human IgM.
About IgM and APC
IgM Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
APC Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm.
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APC Excitation and Emission Spectra