FluoroFinder

	Supplier	Miltenyi Biotec
	Catalog #:	130-115-630 (View supplier product page)
	Size	30 tests in 60 µL
	Price	$155.00
	Antigen	CD25
	Clone	REA945
	Host	Human
	Isotype	IgG1
	Conjugate	Vio Bright 515
	Target Species	Human
	Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
	Description	Clone REA945 recognizes the human CD25 antigen, a 28 kDa glycoprotein also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4+ regulatory T cells. CD25 plays an essential role in the regulation of immune tolerance by controlling regulatory T cells activity. The CD25 antigen contains three epitope regions called A, B, and C. Clone REA945 recognizes epitope region B. \| Additional information: Clone REA945 displays negligible binding to Fc receptors.

	CD25	The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020]
	Vio Bright 515	Vio®Bright® B515 from Miltenyi has an excitation peak at 488 nm and an emission peak at 514. It is comparable to BB515 in flow cytometry.

$155.00
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CD25 / Vio Bright 515 / REA945