CD45 / PerCP / REAL180

Citations: (3)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-121-183 (View supplier product page)
Size 30 µg in 200 µL
Price $125.00
Antigen CD45
Clone REAL180
Host cell line
Isotype IgG
Conjugate PerCP
Target Species Mouse
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Description Clone REAL180 is an antibody fragment derived from the full CD45 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity. | Clone REAL180 recognizes the mouse CD45 antigen (Ly-5, leukocyte common antigen) which is expressed at high levels on all cells of hematopoietic origin except for erythrocytes. Clone REAL180 reacts with all CD45 isoforms. CD45 (Ly-5, leukocyte common antigen) can be used to discriminate leukocytes from non-hematopoietic cells. | The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.
About CD45 and PerCP
CD45 The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
PerCP Peridinin-Chlorophyll-Protein (PerCP) is a red-emitting fluorescent protein isolated from algae that can be excited by the 488 nm blue laser and captured with a 670/30 nm bandpass filter. PerCP exhibits a large Stokes' Shift, with an excitation peak at 482 nm and an emission peak at 675 nm. PerCP is was historically used in flow cytometry, however it is highly susceptible to photobleaching and has poor stability. Alternatives like BB700, NovaFluor Blue 690 or PerCP-eFluor™ 710 are preferred. PerCP is a generic dye that has no sole manufacturer.
Citations
PMID 2479693
PMID 1680920
PMID 8975879
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PerCP Excitation and Emission Spectra