CD235a / Vio Green / REA175

Citations: (1)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-120-617 (View supplier product page)
Size 30 tests in 60 µL
Price $135.00
Antigen CD235a
Clone REA175
Host Human
Isotype IgG1
Conjugate Vio Green
Target Species Human
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC, Mass Cytometry
Description Clone REA175 recognizes CD235a, commonly known as glycophorin A. CD235a is a single-pass transmembrane glycoprotein and is expressed on mature erythrocytes and erythroid precursor cells. It is one of the major sialoglycoprotein expressed on human red blood cells. | The CD235a (Glycophorin A) antibody has been tested by flow cytometric analysis of human peripheral blood cells according to the protocol. Binding of the antibody to red cells at high and moderate antibody concentration can cause cell agglutination, so it is recommended to titrate the antibody carefully for optimal performance in the assay of interest. | Additional information: Clone REA175 displays negligible binding to Fc receptors. |
About CD235a and Vio Green
CD235a Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
Vio Green Vio®Green from Miltenyi has an excitation peak at 388 nm and an emission peak at 520 nm.
Citations
PMID 1391951
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Vio Green Excitation and Emission Spectra