CD235a / PerCP-Vio700 / REA175

Citations: (1)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-120-615 (View supplier product page)
Size 30 tests in 60 µL
Price $160.00
Antigen CD235a
Clone REA175
Host Human
Isotype IgG1
Conjugate PerCP-Vio700
Target Species Human
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC, Mass Cytometry
Description Clone REA175 recognizes CD235a, commonly known as glycophorin A. CD235a is a single-pass transmembrane glycoprotein and is expressed on mature erythrocytes and erythroid precursor cells. It is one of the major sialoglycoprotein expressed on human red blood cells. | The CD235a (Glycophorin A) antibody has been tested by flow cytometric analysis of human peripheral blood cells according to the protocol. Binding of the antibody to red cells at high and moderate antibody concentration can cause cell agglutination, so it is recommended to titrate the antibody carefully for optimal performance in the assay of interest. | Additional information: Clone REA175 displays negligible binding to Fc receptors. |
About CD235a and PerCP-Vio700
CD235a Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
PerCP-Vio700 PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
Citations
PMID 1391951
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PerCP-Vio700 Excitation and Emission Spectra