Arginase 1 Monoclonal / Alexa Fluor 488 / A1exF5

Citations: (18)
Product Details
Supplier Thermo Fisher Scientific
Catalog #: 53-3697-80 (View supplier product page)
Size 25 μg
Price $146.00
Antigen Arginase 1
Clone A1exF5
Host Rat
Isotype IgG2a κ
Conjugate Alexa Fluor 488
Target Species Human, Mouse
Applications FC
Description Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor 488, eBioscience
About Arginase 1 and Alexa Fluor 488
Arginase 1 Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Alexa Fluor 488 Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. Alexa 488 and is similar in size, brightness and application to DyLight™ 488, iFluor® 488 and CF®488A.
Images
  Arginase 1 Alexa Fluor 488 Thermo Fisher Scientific antibody anti target:Human, Mouse host:Rat isotype:IgG2a κ clone:A1exF5 Normal human lysed whole blood cells were stained intracellularly, using the Intracellular Fixati...

Normal human lysed whole blood cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.5 µg of Arginase 1 Monoclonal Antibody, Alexa Fluor 488. Cells were co-stained with either CD16 Monoclonal Antibody, APC (Product # 17-0168-42) or CD193 Monoclonal Antibody, PE (Product # 12-1939-42). Cells in the granulocyte gate (left panel) were used for analysis and were gated on either the CD193+ population (blue histogram) or the CD16+ population (purple histogram).

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Alexa Fluor 488 Excitation and Emission Spectra